TechExchange with Lightcore Technologies and Leica

Double-feature at our next TechExchange webinar!

 The BondXplorer microscope is a turnkey coherent Raman and multiphoton imaging platform designed for label-free, three-dimensional chemical imaging and virtual histology with broad research applications. This versatile system integrates stimulated Raman scattering (SRS), coherent anti-Stokes Raman scattering (CARS), and multiphoton imaging (TPF, SHG) into one high-sensitivity platform. Powerd by the dual color SRS deltaEmerald laser system from APE, this system uniquely features simultaneous dual-channel SRS imaging which is particularly beneficial for obtaining background-free Raman signals in the fingerprint region and which enables the simultaneous acquisition of two signals from both CH2 and CH3 bonds, facilitating lable-free live histological imaging. Additionally, the laser system operates in two regimes: a picosecond source optimized for SRS/CARS imaging and a fixed-wavelength femtosecond source specifically designed for 2P processes, enabling simultaneous coherent Raman and two photon imaging. 

Z-stack and mosaic instantaneous label-free histological image with applied virtual coloring,
allowing to quickly distinguish a tumoral tissue from a healthy one. 
Image source : Histology - Lightcore Technologies

In this webinar, we will present an in-depth overview of our imaging platform, highlighting its key features and capabilities and showcasing its versatility through various applications, such as live virtual histology with an AI-powered coloring scheme, skin imaging, and organoid imaging. 

Julia will be presenting on "Overcoming the high multiplexing barrier: 3D Spatial Omics in one go, powered by Leica Microsystems".

The STELLARIS confocal platform offers significant advancements for high-multiplex strategies, integrating a tunable white light laser (WLL) with an excitation range from 440 nm to 790 nm and up to five highly sensitive, photon-counting spectral detectors. This configuration enables complete flexibility in detection from 410 nm to the near-infrared (NIR) spectrum (1), accommodating a broad array of fluorophores and optimizing their combinations for complex imaging tasks.

In this presentation, we will introduce the new SpectraPlex 3D high-multiplex solution for the STELLARIS confocal platform (2, 3). We will detail the experimental design and considerations for executing high-multiplex experiments with 15+ fluorophores on a single sample, including the selection of optimized panels and the inherent challenges associated with these approaches. We will address the technical aspects of imaging high-multiplex samples in a single round of labeling and imaging, demonstrating its application for 3D spatial omics.

Our aim is to provide a comprehensive understanding of how these technologies can be leveraged to generate detailed spatial omics data, thereby advancing the field of spatial biology.

1. The Power HyD family of detectors. Schweikhard et al., Nature Methods (2022). DOI: d42473-020-00398-0.
2. 3D multiplexing imaging in cancer immunology. Kunz L., Speziale D., et al., Nat. Methods (2024). DOI: d42473-024-00260-7
3. SpectraPlex: A powerful toolbox for advanced 3D high-multiplex imaging. Roberti, M. J., Hecht F. et al., Nat. Methods in press (2024). DOI: d42473-024-00262-5